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Microbiology

Fluorescence microscopy

Bacti pathogenesis

GPC GPR GNC
Clinical bacteria GNR animation microscope
Gram Negative Bacilli

E coli

E coli

Citrobacter diversus

Citrobacter diversus

Achromobacter xyloxidans

Achromobacter xyloxidans

Enterobacter cloacae

Enterobacter cloacae

Morganella morganii

Morganella morganii

Xanthomonas maltophilia

Xanthomonas maltophilia
Strictly anaerobic gram negative bacilli. They are all part of the normal enteric flora. They are all non-invasive organisms which are only pathogenic opportunistically.
Bacteroides fragilis Bacteroides
Fusobacterium Prevotella
The gram negative facultative bacilli represent by far the most numerous group of clinical bacteria. They can be separated into four groups. Those which are fastidious. Those which ferment glucose. Those which do not ferment glucose. Those which are oxidase positive. These distinctions are useful in the accurate identification of the organisms, although automation of the determinations of the biochemical reaction patterns of the gram negative bacilli have made a rigorous knowledge of specific patterns by a technician somewhat less important than in the past for those locations where such automation exists. Where automation exists an isolated colony can be dispersed in a small amount of solution and placed in a machine which, in most instances, will deliver both the antibiotic susceptibility pattern and the id results within about eight hours. Where automation is lacking, a rack of thirty or forty glass tubes must be inoculated individually as well as a disc sensitivity plate and incubated overnight to identify the organismand determine its antibiotic susceptibility pattern.
Antigen-antibody reactions in the form of agglutination procedures, enzyme linked immunoassays, and fluorescent antibody techniques have been developed for the identification of bacterial organisms. These have contributed to increased specificity and rapidity in organism identification. Today it is possible for example to identify Bordatella pertussis or B parapertussis directly from a nasopharyngeal swab by FA microscopic procedure in as little as fifteen minutes. Bordet-Gengou? What's that?
Often the physician is equally if not more concerned with the antimicrobial susceptibility results than the exact genus and/or species identification for a particular organism. In other cases the physician is interested in genus, specie, and serotype or strain as well.
Gram negative bacilli which are fastidious:
Acinetobacter baumannii Actinobacillus actinomycetemcomitans Bordetella pertussis Brucella
Campylobacter Capnocytophaga Cardiobacterium hominis Eikenella corrodens
Francisella tularensis Haemophilus ducreyi Helicobacter pylori Kingella kingii
Legionella pneumophila Pasteurella multocida

Enterobacteriaceae, glucose-fermenting gram-negative bacilli
Citrobacter Enterobacter Escherichia coli Klebsiella pneumoniae
Proteus Salmonella enteriditis Salmonella typhi Shigella
Serratia marcescens Yersinia enterocolitica Yersinia pestis

Gram negative bacilli, non-glucose fermenters
Acinetobacter Pseudomonas aeruginosa Flavobacterium meningosepticum
Xanthomonas maltophilia Burkholderia pseudomallei Burkholderia cepacia
Gram negative bacilli, glucose fermenting, oxidase positive.
Aeromonas Plesiomonas shigelloides Vibrio cholerae Vibrio parahemolyticus
Vibrio vulnificus
Centers for Disease Control
http://www.cdc.gov
National Center for Infectious Diseases
http://www.cdc.gov/ncidod/
Division of Bacterial & Mycotic Diseases
http://www.cdc.gov/ncidod/dbmd/
Morbidity and Mortality Weekly Report, CDC, epidemiological trends, clear perspective.
http://www.cdc.gov/mmwr//
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